FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000771652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31391949
Sequences flagged as poor quality0
Sequence length100
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC576342118.359551361401614TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2349750.7485199469456324TruSeq Adapter, Index 7 (100% over 49bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC892740.28438501859186893TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGATCATCTCGTATGC871180.2775170155889333TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGATCATCTCGTATGC849070.2704738084277596TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGATCATCTCGTATGC782420.24924224997944538TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGATCATCTCGTATGC774960.2468658444877061TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGATCATCTCGTATGC766050.244027537124248TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC757420.24127842460498392TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGATCATCTCGTATGC753760.24011252057016277TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATTATCTCGTATGC742740.23660206634510014TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATCATCTCGTATGC697160.22208241992238198TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC687060.21886503447109956TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCAGATCATCTCGTATGC662420.21101588818203038TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC562630.17922748281733TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)