FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000771653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31391949
Sequences flagged as poor quality0
Sequence length100
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2099750.6688816932010179Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1729940.5510776027318343Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGGAGAGCGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG789670.2515517593380392No Hit
GATCGGGAGAGCGGCGGGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG349390.11129923790332355No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG332210.10582649710599364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)