FastQCFastQC Report
Tue 4 Apr 2023
EGAF00000771662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46345587
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC777909316.78497027127955TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG7395171.5956578562701127TruSeq Adapter, Index 7 (100% over 49bp)
AGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTC2112060.45571976464555297TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCACCAGATCATCTCGTATGCC1854670.4001826538522427Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACCAGATCATCTCGTATG1530940.33033134308990414TruSeq Adapter, Index 23 (97% over 36bp)
AAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTT1333910.2878181260278352TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGATCATCTCGTATGC624290.13470322427893727TruSeq Adapter, Index 7 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)