FastQCFastQC Report
Thu 23 Mar 2023
EGAF00000771666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52266036
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC994030219.018664434394832TruSeq Adapter, Index 5 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2617340.5007726241186533TruSeq Adapter, Index 5 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1568680.30013372355232754TruSeq Adapter, Index 5 (100% over 49bp)
AAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT1000470.1914187638029408TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGATCTCGTATGC707230.1353134949817124TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACCAGATCATCTCGTATG646280.12365200222951671TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC630560.12064431287653038TruSeq Adapter, Index 5 (97% over 46bp)
AGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTC591550.11318057485744662TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACACAGTGATCTCGTATGC531370.10166640531147225TruSeq Adapter, Index 5 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)