FastQCFastQC Report
Thu 16 Mar 2023
EGAF00000771667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52266036
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG715300113.685753784733167Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG1957550.3745357692708894Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1607980.30765294693479334Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG1364830.2611313396715221Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAAAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1138490.21782597019601793Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG941850.1802030672461941Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG828980.15860778116021654Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTATAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG789720.15109621093132067Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCATCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG725480.13880524629799743Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG723310.1383900627168282Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAACGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG660560.1263841780539852Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCATGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG632210.12096000546129039Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCCG550690.10536287848575317Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)