FastQCFastQC Report
Sat 18 Mar 2023
EGAF00000771702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79295220
Sequences flagged as poor quality0
Sequence length100
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC78137979.854058037798495TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1961080.2473137725073466TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1444660.18218752656213072TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGC1264680.1594900676232439TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGC1039180.13105203567125484TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGC989840.12482971861355577TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAATAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC940960.11866541261881863TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGC908810.11461094376180557TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGC869640.10967117563959088TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGC825880.1041525579978213TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)