FastQCFastQC Report
Fri 10 Mar 2023
EGAF00000771704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78838971
Sequences flagged as poor quality0
Sequence length100
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC77203699.792579611420853TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1927780.24452120259154572TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1424400.18067206889344103TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGC1251350.15872226439891002TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAATAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1167420.1480765140884449TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGC1010860.1282183147722717TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGC976150.12381566979102251TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGC913500.11586909220314405TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGC898650.11398550597521116TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGC822610.10434052976160736TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)