FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000771712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80408314
Sequences flagged as poor quality0
Sequence length100
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC78279959.735305480973025TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1951250.24266769229858495TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1451110.18046765661570768TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGC1256320.15624254974429636TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGC1024370.12739603021647736TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGC995860.1238503769647502TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAATAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC948340.1179405403277079TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGC938950.1167727506386964TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGC916010.11391981182443398TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGC837100.104106150018268TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)