FastQCFastQC Report
Tue 5 Sep 2023
EGAF00000771942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43893746
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1545016235.19900534349472TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC42294969.635759955415972TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAATAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1327590.3024553885193576TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTAACCGATGTATCTCGTATG1262190.28755577161265755TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTTAACTCCAGTCACCGATGTATCTCGTATG1221180.27821275495602493TruSeq Adapter, Index 2 (97% over 49bp)
AGATCTGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG699040.15925731196421467TruSeq Adapter, Index 2 (97% over 49bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG687200.15655988896459191TruSeq Adapter, Index 2 (97% over 49bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG669720.1525775448739326TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTACAGTCACCGATGTATCTCGTATG619810.1412069045098133TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCAAGTCACCGATGTATCTCGTATG498250.11351275418598357TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGTAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG485490.11060573412895768TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATGTATCTCGTATG466600.10630215976553925TruSeq Adapter, Index 2 (97% over 49bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG458910.10455020175311536TruSeq Adapter, Index 2 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)