FastQCFastQC Report
Wed 15 May 2024
EGAF00000772162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000772162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36849165
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC1065240.2890811772804079TruSeq Adapter, Index 13 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT955210.2592216132984289TruSeq Adapter, Index 13 (97% over 40bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC655100.17777879091697193TruSeq Adapter, Index 13 (97% over 34bp)
AGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT398450.10812999426174241TruSeq Adapter, Index 13 (96% over 33bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG379410.10296298437155903TruSeq Adapter, Index 13 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)