FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000772167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000772167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20645920
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1670790.8092591659756504Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC663590.3214145942636608Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG505500.24484256453575332Illumina Single End PCR Primer 1 (98% over 50bp)
TCTGCTTGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT272230.13185656052140082Illumina Single End PCR Primer 1 (97% over 43bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGTCGCCG251440.12178677433604315Illumina Single End PCR Primer 1 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)