FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000772574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000772574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16541486
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT4117262.489050862782219No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA3770112.2791845907919033RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC3613342.1844107597104636RNA PCR Primer, Index 1 (100% over 26bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA3470772.0982214052594794RNA PCR Primer, Index 1 (100% over 28bp)
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA3274251.979417085018843No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA2136741.2917460982646904RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC2136051.2913289652453233No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1813611.0964008916732149RNA PCR Primer, Index 1 (100% over 30bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA1572530.9506582419499674RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1568220.9480526719304421No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1546370.9348434596504812RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1545550.9343477363521029RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT1502170.9081227647866704No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1489820.9006566882806054No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT1453610.878766272872945No Hit
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT1393570.8424696547819223No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1277640.7723852621221575RNA PCR Primer, Index 1 (100% over 27bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT1243640.7518308814576877No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1242960.7514197938443983RNA PCR Primer, Index 1 (100% over 26bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC1213590.7336644361939428RNA PCR Primer, Index 1 (100% over 27bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC1084550.6556545161662017No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1020520.6169457810501426RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA997130.6028055762342029RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG949190.5738238994973003No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG935310.5654328758613343Illumina Small RNA Adapter 2 (100% over 21bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC933310.5642237946457773No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA931740.563274665891565RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG884860.5349338021989076RNA PCR Primer, Index 1 (100% over 29bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG864340.5225286289272922No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA859580.5196510156342665RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC843960.5102080913407658RNA PCR Primer, Index 1 (100% over 27bp)
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG801220.48437002576431165No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA785540.4748908290343443RNA PCR Primer, Index 1 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT782320.47294420827729744No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC775970.4691053754179038No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG718550.4343926537192608No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA675340.4082704540571506RNA PCR Primer, Index 1 (100% over 28bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA674410.4077082312919166No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC663640.40119732894614185RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG585180.3537650728598386No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG562930.3403140443367664Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC562540.34007827349973274RNA PCR Primer, Index 1 (100% over 26bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC535810.3239189030538127No Hit
AATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGG520230.31450016038462325No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT509430.30797112182061515No Hit
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC494020.29865515105474805RNA PCR Primer, Index 1 (100% over 27bp)
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGTGGAATTCTCGGG486780.2942782770544315No Hit
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCTGGAATTCTCG468440.28319100230777333No Hit
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG467520.2826348249486171No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA459690.2779012719897112No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG454670.274866478138663Illumina Small RNA Adapter 2 (100% over 21bp)
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG448790.27131177936492523No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT435520.2632895254997042No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA434750.2628240292317148No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA432950.2617358561377134RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT424760.25678466856000726No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG415720.25131962146568937No Hit
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC413040.2496994526368429RNA PCR Primer, Index 1 (100% over 27bp)
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTGGAATTCT405460.24511703482988167No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA400880.242348238846256RNA PCR Primer, Index 1 (100% over 22bp)
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT399610.2415804722743773No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC382010.23094055757747523No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG352160.21289502043528616No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT351940.2127620215015749No Hit
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTC346600.20953377465603756No Hit
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA342450.20702493113375664RNA PCR Primer, Index 1 (100% over 23bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA337780.20420172649543095No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC326160.19717696463304443No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG295150.17843016038583231No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTGGAATTC294100.17779539274766487No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA293160.17722712457635306RNA PCR Primer, Index 1 (100% over 28bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG291820.17641704016192983Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT286850.17341247334127055RNA PCR Primer, Index 1 (100% over 25bp)
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCA281300.17005727296809972No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG281030.16989404700399952RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC274340.16584967033796116No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC273330.16523908432410486RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC273170.1651423578268603RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG272260.16459222587378183Illumina Small RNA Adapter 2 (100% over 21bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC269640.1630083294814021RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA267900.16195642882386746RNA PCR Primer, Index 1 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG266130.16088639194809948No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA264320.15979217344802032RNA PCR Primer, Index 1 (100% over 22bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC260850.15769441753902885No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA257330.15556643459964842RNA PCR Primer, Index 1 (100% over 22bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG250230.15127419628442088No Hit
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG246010.1487230349195955RNA PCR Primer, Index 1 (100% over 29bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG245570.14845703705217295Illumina Small RNA Adapter 2 (100% over 21bp)
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT245410.14836031055492838No Hit
TAATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCG244120.14758045317089408No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA235190.14218190554343182RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA235030.14208517904618728RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG233040.140882143236708RNA PCR Primer, Index 1 (100% over 29bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG230950.13961865336645085No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG226640.1370130833469254RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC224900.13596118268939078RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG223720.13524782477221214RNA PCR Primer, Index 1 (100% over 29bp)
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGAATTCTCGGGTGCC221070.13364579216159903No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA218290.1319651692719747RNA PCR Primer, Index 1 (100% over 28bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA209600.12671171139037932No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA204730.12376759863049788RNA PCR Primer, Index 1 (100% over 23bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC203820.12321746667741944No Hit
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT203330.12292124177960796RNA PCR Primer, Index 1 (100% over 30bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA202690.1225343357906297RNA PCR Primer, Index 1 (100% over 28bp)
CTCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGG197080.11914286298099215No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG197070.11913681757491437Illumina Small RNA Adapter 2 (100% over 21bp)
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTTGGAATTCTCG194220.11741387684274557No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTGGAATTCTCGGGTGCCAA193230.11681538164104482No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCT193200.11679724542281147No Hit
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATTC185800.11232364492525036No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC182780.11049793228975922No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC182270.11018961657979216RNA PCR Primer, Index 1 (100% over 26bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA181210.10954880353554694RNA PCR Primer, Index 1 (100% over 32bp)
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACA180290.10899262617639068No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC175170.10589737826456462No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA174640.105576971742442No Hit
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGG173680.10499661275897461Illumina Small RNA Adapter 2 (100% over 21bp)
CACCATGATGGAACTGAGGATCTGAGGTGGAATTCTCGGGTGCCAAGGAA171900.10392053047712883RNA PCR Primer, Index 1 (100% over 23bp)
TTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA167240.1011033712448809No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)