FastQCFastQC Report
Fri 6 Jan 2023
EGAF00000772708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000772708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146372002
Sequences flagged as poor quality0
Sequence length100
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT97062826.63124222349572TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAACTCGTAT1546380.10564725349592471TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA1497330.10229620279430214TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)