FastQCFastQC Report
Tue 24 Jan 2023
EGAF00000772710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000772710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122239404
Sequences flagged as poor quality0
Sequence length100
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT105089948.597059259222174TruSeq Adapter, Index 18 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG2673330.21869625607795012TruSeq Adapter, Index 18 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA1581800.12940180892897676TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1556490.12733128181809525TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTCCGCACATCTCGTAT1529380.1251135026803632TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTCCGCACATCTCGTAT1342730.10984428556277974TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)