FastQCFastQC Report
Mon 18 Sep 2023
EGAF00000772716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000772716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31945660
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC21252646.652747196332773TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG13476294.218504172397753TruSeq Adapter, Index 10 (100% over 49bp)
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC2003710.6272244805710697TruSeq Adapter, Index 10 (100% over 42bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGT482990.15119111641456148TruSeq Adapter, Index 10 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCACTAGCTTATCTCGTATGCC337540.10566067503379176Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)