FastQCFastQC Report
Mon 18 Sep 2023
EGAF00000772717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000772717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31945660
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC12789584.003542265209108Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7354652.302237612245294Illumina Single End PCR Primer 1 (100% over 50bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1885200.5901271095979861Illumina Single End PCR Primer 1 (100% over 43bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG1258370.393909532625089Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCCG500670.15672551451433467Illumina Single End PCR Primer 1 (98% over 50bp)
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC462540.14478962087494826Illumina Single End PCR Primer 1 (100% over 47bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGTCGCCG377300.11810681012694682Illumina Single End PCR Primer 1 (97% over 41bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)