FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000772730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000772730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20570224
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA7879143.8303617889625317RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA6668293.2417196818080347RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC5273602.563705674765622RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4948082.4054575195680905RNA PCR Primer, Index 1 (100% over 30bp)
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA3260231.5849268340490603No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2890531.4052010323271151RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2807811.3649875664941713RNA PCR Primer, Index 1 (100% over 28bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA2756371.3399805466386754RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC2535921.2328110768263874No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA2375561.1548537342131033RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC2359761.1471727288920142RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC2147291.0438826529064535RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC2024470.9841749900244159RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1992040.9684094835330913RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA1904350.9257799040010454RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1789290.8698446842387327RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1728030.8400637737343064RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1651080.8026553332622921No Hit
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1552720.7548386444406244RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1547390.7522475204937RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1388920.6752089816814829RNA PCR Primer, Index 1 (100% over 28bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT1304000.633926008778514No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG1288270.6262790332278345Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA1181400.5743252966034789RNA PCR Primer, Index 1 (100% over 28bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG1079610.5248411490317266Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1078420.5242626429347585RNA PCR Primer, Index 1 (100% over 28bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT1008470.49025717950373315No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA994010.48322760121620456RNA PCR Primer, Index 1 (100% over 32bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG942080.45798237296783934No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC936570.4553037438970038RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA841400.40903784032687246RNA PCR Primer, Index 1 (100% over 28bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG826220.4016582415436992Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC809000.39328691802286647RNA PCR Primer, Index 1 (100% over 26bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC801040.3894172469876847RNA PCR Primer, Index 1 (100% over 27bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC784760.381502894669499No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC743410.3614010231487999No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA735230.3574244014066157RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC701300.3409296855493649RNA PCR Primer, Index 1 (100% over 27bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA699980.3402879813073499No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG677840.3295248510662791RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT665050.323307125872815No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC654000.3179352835438253RNA PCR Primer, Index 5 (100% over 35bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA649920.315951834068506RNA PCR Primer, Index 1 (100% over 28bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC629320.3059373587764528RNA PCR Primer, Index 1 (100% over 33bp)
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG624860.3037691762617656No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC593860.28869884936595736No Hit
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG572520.2783246307867139RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC558380.27145061716391616RNA PCR Primer, Index 1 (100% over 27bp)
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG554070.2693553555858215No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT540840.2629237289783524No Hit
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG534970.2600700896596945RNA PCR Primer, Index 1 (100% over 29bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT527090.25623930979069554No Hit
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC509890.24787770906140838RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG503230.24464001947669603RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA497380.2417961029495838No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA488330.23739653977516237No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG474760.23079962571141666Illumina Small RNA Adapter 2 (100% over 21bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA458980.22312834318187297RNA PCR Primer, Index 1 (100% over 23bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC455260.221319903954376RNA PCR Primer, Index 1 (100% over 33bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG453970.22069278389967947Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA450400.2189572656087751RNA PCR Primer, Index 1 (100% over 22bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA444480.21607931931125302RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC437860.212861075309632RNA PCR Primer, Index 1 (100% over 26bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA435980.21194713290433784RNA PCR Primer, Index 1 (100% over 22bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT433640.21080956629349296RNA PCR Primer, Index 1 (100% over 30bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC433360.21067344721185344No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG432260.21013869367684088RNA PCR Primer, Index 1 (100% over 29bp)
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG424090.20616693333042946No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG422720.20550092210955018RNA PCR Primer, Index 1 (100% over 29bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA418680.20353691821732225RNA PCR Primer, Index 1 (100% over 22bp)
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG407380.19804354099401153No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG389510.18935622674794403RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT387960.1886027104031536RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT384400.18687205350802208No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC383590.18647828045042192RNA PCR Primer, Index 1 (100% over 33bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT383420.18639563672228363No Hit
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC381460.18544280315080672RNA PCR Primer, Index 1 (100% over 27bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA378970.18423231560336922RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA377480.18350796763321586RNA PCR Primer, Index 1 (100% over 28bp)
AATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGG366150.17800000622258658No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG352390.17131072563915686RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG350610.1704453971915911RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC349240.16977938597071185RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG345820.16811678861640011Illumina Small RNA Adapter 2 (100% over 21bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG343830.16714937085760467Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGG341900.16621112147344627Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC339980.16527773348506072RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC333030.16189906342293597RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA324810.1579029960976604RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA323020.15703280625432178RNA PCR Primer, Index 1 (100% over 28bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG316870.15404304785402434No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC316580.15390206737661194RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC305240.14838924457020983No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCA302300.14695999421299447No Hit
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCTGGAATTCTCG301780.1467072016328067No Hit
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGTGGAATTCTCGGG291730.14182149888110115No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG272840.1326383222662038No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT265050.1288512949591604No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA264710.12868600750288378RNA PCR Primer, Index 1 (100% over 23bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG260040.12641573567696687RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC251120.12207937064759238No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGAATTCTCGGGTGCC247280.12021259467082128No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT246690.11992577232022364No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA243530.11838957125600577RNA PCR Primer, Index 1 (100% over 32bp)
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCC240410.11687281577487928RNA PCR Primer, Index 1 (100% over 27bp)
CACCATGATGGAACTGAGGATCTGAGGTGGAATTCTCGGGTGCCAAGGAA240320.1168290632129237RNA PCR Primer, Index 1 (100% over 23bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC236790.11511299050511069RNA PCR Primer, Index 1 (100% over 27bp)
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG234320.11391222574921886No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACA234310.11390736435344603No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA231030.11231282653995406No Hit
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC230640.11212323210481326RNA PCR Primer, Index 1 (100% over 27bp)
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCG229450.11154472600784512No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC228840.1112481808657018RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC228360.1110148338686054RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC227430.11056272406173118RNA PCR Primer, Index 1 (100% over 27bp)
TGGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAG226180.109955049590126No Hit
TGAGGTAGTAGTTTGTACAGTTATGGAATTCTCGGGTGCCAAGGAACTCC218570.10625552740699373RNA PCR Primer, Index 1 (100% over 27bp)
TGAGAACTGAATTCCATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA216270.1051374063792402RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTAATGGAATTCTCGGGTGCCAAGGAACTCCA211530.10283310478291341RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCGTGAGGATAAATAACTCTGAGGTGGAATTCTCGGGTGCCAAGGAA211300.10272129268013805RNA PCR Primer, Index 1 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC211230.10268726290972816No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC210660.1024101633506762No Hit
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT209500.10184624144102661RNA PCR Primer, Index 1 (100% over 30bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTGGAATTCTCGGGTGCCAA207860.10104897253428062No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)