FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000772853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000772853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5095
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGCACTGACCAATCTCGTATGCC4528.871442590775269Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTGACCAATCTCGTATGC541.0598626104023552TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTACTGACCAATCTCGTATGCC380.745829244357213Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCATTGACCAATCTCGTATG170.3336604514229637TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTACTGACCAATCTCGTATGC150.2944062806673209Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCACTGACCAATCTCGTATGC80.15701668302257116Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)