FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000772861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000772861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18641546
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA6755953.6241361097411127RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA5231102.8061513782172356RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4845712.59941423313281RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC4656362.4978400396619462RNA PCR Primer, Index 1 (100% over 26bp)
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA3947002.117313660573002No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA3488791.8715132317888226RNA PCR Primer, Index 1 (100% over 28bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA2185861.1725744205979483RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2170781.1644849627815204RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1940141.0407613188305305RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1938911.0401015023110207RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1919361.0296141747041796RNA PCR Primer, Index 1 (100% over 27bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT1827530.9803532389427357No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1730260.928174090281997RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1710640.9176492121415253RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC1674400.8982087644447516RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA1670420.8960737483897526RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1663110.8921523998063251No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1652360.8863857107130493No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG1555990.8346893546275614No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1508720.8093320157029894RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1497100.8030986271203043RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT1444630.7749518199831709No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1407700.7551412313120381RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1360590.7298697221786219RNA PCR Primer, Index 1 (100% over 29bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC1219680.6542804979801569No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1078660.5786322658002722RNA PCR Primer, Index 1 (100% over 32bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC956260.5129724755661359RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA924930.4961659295854539RNA PCR Primer, Index 1 (100% over 28bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT881720.4729865216114586No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG858370.4604607364646688No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG819550.4396362833855089Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA814200.43676634974373907RNA PCR Primer, Index 1 (100% over 28bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG782600.4198149659904816Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA697260.3740355011327923No Hit
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC668720.35872561213538834No Hit
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC657080.3524814948288087RNA PCR Primer, Index 1 (100% over 27bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC652310.3499226941799784RNA PCR Primer, Index 1 (100% over 26bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT611100.3278161585954298No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA606000.32508033400234077No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA589020.3159716474159386RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG578800.3104892695058661RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC576780.309405668392525RNA PCR Primer, Index 1 (100% over 27bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG565470.30333857503020406Illumina Small RNA Adapter 2 (100% over 21bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC551470.29582846830407733No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA507050.27199997253446684RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA507000.27197315072473066No Hit
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC505370.2710987597273316RNA PCR Primer, Index 1 (100% over 33bp)
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG495140.2656110174553119No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA487720.2616306608904648RNA PCR Primer, Index 1 (100% over 28bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC484530.2599194294292973No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT480860.2579507085946627No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCA471930.2531603333757833No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA469530.2518728865084473No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG465350.24963058321450377RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG459000.2462242133780106RNA PCR Primer, Index 1 (100% over 29bp)
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG455150.24415893402832578No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC445660.2390681545404013RNA PCR Primer, Index 5 (97% over 35bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT438290.23511461978529036No Hit
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG432800.23216958507625923RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG430560.23096796800007893RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC418930.22472921505544657RNA PCR Primer, Index 1 (100% over 27bp)
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG414190.222186507492458No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG413910.22203630535793545RNA PCR Primer, Index 1 (100% over 29bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG399950.2145476560795977Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC399230.2141614220193969RNA PCR Primer, Index 1 (100% over 26bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT397050.2129919911149RNA PCR Primer, Index 1 (100% over 30bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA394320.21152752030330532RNA PCR Primer, Index 1 (100% over 22bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC383730.2058466610011852RNA PCR Primer, Index 1 (100% over 27bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT380590.20416225134975396No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA375270.20130841079382578RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA364650.19561145840586397RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC358710.19242502740920736No Hit
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCTGGAATTCTCG354780.19031683316394465No Hit
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG346160.18569275316542952RNA PCR Primer, Index 1 (100% over 29bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG345860.1855318223070125No Hit
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA341740.18332170518475238RNA PCR Primer, Index 1 (100% over 23bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA341530.18320905358386047RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT335530.17999043641552046RNA PCR Primer, Index 1 (100% over 25bp)
AATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGG333360.17882636987297085No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC331290.17771594694989354RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG329410.17670744690381365RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC326860.17533953460726917No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG325330.17451878722934247Illumina Small RNA Adapter 2 (100% over 21bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA311940.167335906581997RNA PCR Primer, Index 1 (100% over 22bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC310750.16669754751027624RNA PCR Primer, Index 1 (100% over 27bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC308850.16567831874030192No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA303050.16256698881090656RNA PCR Primer, Index 1 (100% over 22bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG302210.16211638240733897Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC291200.15621021990343506RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG288140.15456872514758166RNA PCR Primer, Index 1 (100% over 29bp)
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCG279760.1500733898358001No Hit
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA272350.1460983976329002RNA PCR Primer, Index 1 (100% over 28bp)
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG267780.14364688422301455No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA267690.14359860496548946No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT266410.1429119666362436No Hit
TTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA266170.14278322194951No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACA264670.14197856765742498No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC260900.13995620320331803RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC257710.13824497174215056RNA PCR Primer, Index 1 (100% over 27bp)
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGAATTCTCGGGTGCC244880.1313624953638502No Hit
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG244600.13121229322932768Illumina Small RNA Adapter 2 (100% over 21bp)
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTC241280.1294313250628462No Hit
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGTGGAATTCTCGGG240860.1292060218610624No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG239850.1286642213043918RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT235820.12650238343965678No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC235500.1263307238573453RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTT234660.12588011745377772No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG233890.12546706158384074No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA232940.12495744719885357RNA PCR Primer, Index 1 (100% over 23bp)
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT232290.1246087636722834No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC229830.12328913063326401No Hit
TCTCACACAGAAATCGCACCCGTTGGAATTCTCGGGTGCCAAGGAACTCC224030.12017780070386866RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA222000.1190888352285803RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC219220.11759754260724942RNA PCR Primer, Index 1 (100% over 26bp)
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG218420.11716839365147075No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC215870.11580048135492625No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTGGAATTCTCGGGTGCCAA213800.11469005843184894No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC212230.11384785360613332No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA210000.11265160089190028No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT209430.11234583226090798No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC202750.1087624384801561RNA PCR Primer, Index 1 (100% over 26bp)
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATTC201410.10804361397922682No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGG199740.10714776553403886Illumina Small RNA Adapter 2 (100% over 21bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA197870.10614462984990622RNA PCR Primer, Index 1 (100% over 32bp)
TGAGAACTGAATTCCATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA196290.10529706066224336RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTAATGGAATTCTCGGGTGCCAAGGAACTCCA191780.10287773342404112RNA PCR Primer, Index 1 (100% over 28bp)
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTGGAATTCT187600.10063543013009758No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG187530.10059787959646695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)