FastQCFastQC Report
Tue 14 Feb 2023
EGAF00000772982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000772982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58082857
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC668361711.507038987424464TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG880740.15163510293579394TruSeq Adapter, Index 7 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)