FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000773130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000773130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64923903
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1112130517.129754198542255TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA929235714.312690042679657TruSeq Adapter, Index 13 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1767410.272227934294092TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT1280820.19728019124173726TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTA1184410.18243049867165256TruSeq Adapter, Index 13 (97% over 40bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA954210.1469736038512657TruSeq Adapter, Index 13 (97% over 40bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT759040.11691225649203499TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCACAGTCAACAATCTCGTATG662570.10205332233337852Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)