FastQCFastQC Report
Tue 14 May 2024
EGAF00000773168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000773168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57622022
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC3304110.5734109781846948TruSeq Adapter, Index 13 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1656590.28749251458062336TruSeq Adapter, Index 13 (97% over 40bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG1655920.28737623959117575TruSeq Adapter, Index 13 (96% over 29bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC1419600.24636414182063934TruSeq Adapter, Index 13 (97% over 34bp)
AGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT975180.16923737941719574TruSeq Adapter, Index 13 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)