FastQCFastQC Report
Fri 10 Feb 2023
EGAF00000773198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000773198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101045534
Sequences flagged as poor quality0
Sequence length100
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT80174617.934503072644457TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA7448450.7371379718771143TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTCAACAATCTCGTAT1473550.14583029468674982TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTCAACAATCTCGTAT1472330.14570955703989846TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTCAACAATCTCGTAT1408140.13935697544039896TruSeq Adapter, Index 13 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1407640.13930749279824678TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTAACAATCTCGTAT1364200.13500844084806363TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTCAACAATCTCGTAT1347280.13333394823763314TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1317320.1303689483198733TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1292390.1279017437821646TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACAGTCAACAATCTCGTAT1276320.12631137166339285TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATAGTCAACAATCTCGTAT1220710.12080791220322515TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1182420.11701853146720964TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTAT1095460.10841251034409893TruSeq Adapter, Index 13 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)