FastQCFastQC Report
Thu 14 Sep 2023
EGAF00000773244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000773244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60105778
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT32122105.344261578312821TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA27927104.646325349952213TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT765090.12729059093120798TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTA690090.11481258923226982TruSeq Adapter, Index 15 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)