FastQCFastQC Report
Tue 27 Sep 2022
EGAF00000773256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000773256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27549157
Sequences flagged as poor quality0
Sequence length100
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC18817886.830655471599368TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC503380.18272065457393125TruSeq Adapter, Index 12 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG486760.17668780209862683TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGC315350.11446811239995475TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGC310970.11287822709057849TruSeq Adapter, Index 12 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC310190.11259509683000464TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGC299340.10865668230791961TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGC298710.10842800017437919TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC291920.10596331495733245TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC287870.10449321552742975TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGC277730.10081252213996965TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)