FastQCFastQC Report
Tue 27 Sep 2022
EGAF00000773258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000773258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27544316
Sequences flagged as poor quality0
Sequence length100
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC18895776.860134047256791TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC495640.17994275116506797TruSeq Adapter, Index 12 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG482870.17530658593954557TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGC383950.13939355037896023TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGC315950.11470606131588092TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGC314900.1143248574406422TruSeq Adapter, Index 12 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC306560.11129700951731747TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGC302640.1098738483830929TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC288580.1047693469679915TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC283200.10281613092153023TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)