FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000773266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000773266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149305576
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC145033919.713897758245814TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2655120.17783126867277885TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGC2465100.1651043494852463TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGC2374810.15905702008075034TruSeq Adapter, Index 12 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2259060.15130446300277492TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGC2209380.14797705880723436TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGC2205600.147723886748878TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGC2204700.1476636076873646TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2180780.14606152418580803TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGC2083650.13955607391381014TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCTTGTAATCTCGTATGC2079310.13926539488384546TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2065790.13835986942644393TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1930340.12928787066867484TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)