FastQCFastQC Report
Tue 5 Sep 2023
EGAF00000773322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000773322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63040185
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC53750958.526458163154185TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG35817835.681745699191714TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC955640.15159219472468236TruSeq Adapter, Index 12 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATG691780.10973635308969985TruSeq Adapter, Index 12 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)