FastQCFastQC Report
Thu 23 Mar 2023
EGAF00000776271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000776271
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences58379413
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA3777190.6470071907026541No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA1840830.31532177276260037No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACC993810.1702329552371484No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC973110.16668718474438926No Hit
CAAAATAATCTCGATCTCCTAACCTCGTAATCCGCCCGCCTCGACCTCCC760850.1303284772664638No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA722140.12369771515174365No Hit
CATCAGCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG685990.11750546378395411Illumina Single End PCR Primer 1 (100% over 41bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC675080.11563665431168348No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCTAAATAACTAAAACTACA630870.10806377926410463No Hit
CAAATAATCAATACTATATCCGACAATATTCGTATTTTATTTTCTTCCTA593670.10169166997276934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)