FastQCFastQC Report
Thu 23 Mar 2023
EGAF00000776273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000776273
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences57258839
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA2468180.43105659197875107No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACC1379340.24089555850058364No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA1323900.2312132105926912No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC902690.15765076899306324No Hit
CATCAGCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG854070.1491595035659036Illumina Single End PCR Primer 1 (100% over 41bp)
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCTTATTAACC798240.13940904390324785No Hit
CAAATAATCAATACTATATCCGACAATATTCGTATTTTATTTTCTTCCTA712220.12438603583981157No Hit
CAAAATAATCTCGATCTCCTAACCTCGTAATCCGCCCGCCTCGACCTCCC644700.11259396998950677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)