FastQCFastQC Report
Fri 10 Feb 2023
EGAF00000780454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000780454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106688286
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACTCCAGTCACCAATTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA7139350.6691784325788119Illumina PCR Primer Index 11 (96% over 28bp)
GAACTCCAGTCACGATGCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA2044650.19164709422738313TruSeq Adapter, Index 4 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)