FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212364
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTTGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC4849622.8362622666742No Hit
TGTTCCTCAAAGTTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC4686422.067770431899945No Hit
TAGTTGGGATTGCGAACAGCTTTTTCTTTTTCATCTGCAGCACTGCCTCC179318.443521500819347No Hit
TAGTTGGGATTGCGAACACCTTTTTCTTTTTCATCTGCAGCACTGCCTCC122095.749091183063042No Hit
TGTTCCTCAAAGGTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC58262.743402836638978No Hit
TGTTCCTCAAAGTATTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC50322.3695164905539543No Hit
TGTTCCTCAATGTTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC31571.4865984818519147No Hit
TGTTCCTCAAAGTTTTCCTATAGCAGATTTTTCTTACATTTAGTTTTAAC29401.3844154376447986No Hit
TGTTCCTCAAAGTTTACCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC29231.3764103143659001No Hit
TGTTCCTCTAAGTTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC21681.0208886628618785No Hit
TGTTCCTCAAAGTTTTCCTCTACCAGATTTTTCTTACATTTAGTTTTAAC15840.7458891337514834No Hit
TGTTCCTCAAAGTTTTCCTCTTGCAGATTTTTCTTACATTTAGTTTTAAC13930.6559492192650355No Hit
TGTTTCTCAAAGTTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC8660.4077903976191822No Hit
TAGGTGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC7580.3569343203179447No Hit
TGTTCCTCAAAGGATTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC7390.347987417829764No Hit
TAGTTGGGAGTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC7160.33715695692301895No Hit
CAGTTGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC6510.30654913262134825No Hit
TGTTCCTCAAAGTTTTCCTCAAGCAGATTTTTCTTACATTTAGTTTTAAC6090.28677176922642256No Hit
TAGTTGGGATGGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC5990.2820628731800117No Hit
TAGCTGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC5640.2655817370175736No Hit
TAGTTGGGATGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCCT4910.23120679587877416No Hit
AGTTCCTCAAAGTTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC4880.22979412706485092No Hit
GAGTTGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC4780.22508523101844005No Hit
TAGTCGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC4750.22367256220451678No Hit
TGTTCCTCAATGGTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC4630.21802188694882374No Hit
TAGTTGGGACTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC4580.21566743892561827No Hit
TAGTTGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCCT4540.21378388050705394No Hit
TAGTGGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC4360.20530786762351433No Hit
TAGTTGGGATTGCGAACAGCACACGTCTGAACTCCAGTCACACAGTGATC4120.1940065171121282TruSeq Adapter, Index 5 (97% over 37bp)
TAGTTGGGATTGAGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC4070.1916520690889228No Hit
TACTTGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC4050.19071028987964062No Hit
TAGTTCGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC4050.19071028987964062No Hit
TAGTTGGGATCGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC3970.18694317304251193No Hit
TGGTTGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC3960.18647228343787084No Hit
TAGTGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCCT3890.18317605620538321No Hit
TGTTCCCCAAAGTTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC3740.17611271213576687No Hit
TGTTCCTCAAAGGTTTCCTATAGCAGATTTTTCTTACATTTAGTTTTAAC3700.17422915371720255No Hit
TGTTCCTCAATGTATTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC3700.17422915371720255No Hit
TAGTTGGGATTGCGGACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC3570.16810758885686838No Hit
TGTTCCTCAAAGTATTCCTATAGCAGATTTTTCTTACATTTAGTTTTAAC3510.16528225122902188No Hit
TGTTCCTCAAAGTATACCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC3470.16339869281045752No Hit
TGTTCCTCAAAGGTTACCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC3400.1601024655779699No Hit
TAGTTGGGATTGCCAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC3380.15916068636868771No Hit
TAGTTGGGATTGCGAAAAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC3310.15586445913620012No Hit
TAGTTGGGGTTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC3290.15492267992691794No Hit
AGTTGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCCT3270.15398090071763576No Hit
TAGTTGAGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC3220.1516264526944303No Hit
TCGTTGGGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC3030.14267955020624964No Hit
TAGTTGGGATTGGGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC3030.14267955020624964No Hit
TAGTTGGGATTGCGAGCAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC2980.1403251021830442No Hit
TGTTCCTCAAAGTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAACA2850.13420353732271006No Hit
TAGTTGGCATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC2810.1323199789041457No Hit
TAGGTGGGATTGCGAACAGCTTTTTCTTTTTCATCTGCAGCACTGCCTCC2710.12761108285773481No Hit
TAGTTGGGCTTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC2690.12666930364845264No Hit
TGTTCCTCTAAGTATTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC2670.12572752443917048No Hit
TAGTTGGGATTGCGCACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC2640.12431485562524722No Hit
TAGTTGGGATTACGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC2640.12431485562524722No Hit
TAGTTGGGATTGCGAACATCTTTTTCTTTTTCATCTGCAGCACTGCCTCC2630.12384396602060613No Hit
TAGTTGGGAGTGCGAACAGCTTTTTCTTTTTCATCTGCAGCACTGCCTCC2610.12290218681132395No Hit
TAGTTGGGATTGCGAACCACTTTTTCTTTTTCATCTGCAGCACTGCCTCC2570.1210186283927596No Hit
TGTCCTCAAAGTTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAACA2570.1210186283927596No Hit
TAGTTGGGATTGCGAACGACTTTTTCTTTTTCATCTGCAGCACTGCCTCC2530.11913506997419523No Hit
TAGTTGGGATTGCGAAGAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC2480.1167806219509898No Hit
TGTTCCTCAAAGTTATCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC2480.1167806219509898No Hit
GTTCCTCAAAGTTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAACA2470.11630973234634873No Hit
TAGTTGGAATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC2420.11395528432314328No Hit
TGTTCCTCTAAGGTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC2380.11207172590457894No Hit
CAGTTGGGATTGCGAACAGCTTTTTCTTTTTCATCTGCAGCACTGCCTCC2240.1054792714396037No Hit
TGTTCCTCAATGTTTTCCTATAGCAGATTTTTCTTACATTTAGTTTTAAC2200.10359571302103936No Hit
TGTTCGTCAAAGTTTTCCTCTAGCAGATTTTTCTTACATTTAGTTTTAAC2130.10029948578855173No Hit
TAGTTGCGATTGCGAACAACTTTTTCTTTTTCATCTGCAGCACTGCCTCC2130.10029948578855173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)