FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212364
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATGAAGTTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC8054137.92591964739786No Hit
CAAAGGAGGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA4893023.040628355088433No Hit
CAAAGGAGGCTCTAGGTTTAGTCTATCATCTCAGTTCAGAGGCAACGAAA32111.5120265205025334No Hit
CAAAGGAGGCTCAAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA25521.2017102710440564No Hit
AAAATGAAGTTTCTGTAGCCTAACCTCGAGATATCTCCCCAGTACCATTC23851.1230717070689948No Hit
CCAAGGAGGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA21561.0152379876061857No Hit
AAAATGAAGTTTCTGAAGCCTTACCTCGAGATATCTCCCCAGTACCATTC21451.0100582019551336No Hit
CAAAGGACGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA20960.9869846113277203No Hit
AAAATGAAGTTTCGGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC16890.7953325422387976No Hit
CAAAGGAGGCTCTAGGATTTGTCTATCATCTCAGTTCAGAGGCAACGAAA16130.7595449322860748No Hit
CAAAGGAGGGTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA15320.7214028743101467No Hit
CAAAGGAGGCTCTCGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA15160.7138686406358894No Hit
AAAATGAAGTTTCTGTAGCATTACCTCGAGATATCTCCCCAGTACCATTC14840.6988001732873745No Hit
AAAATGAAGTTCCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC14340.6752556930553201No Hit
CACAGGAGGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA12430.5853157785688723No Hit
AACATGAAGTTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC11100.5226874611516076No Hit
AAAATGAAGTTTCTGTAGCCCTACCTCGAGATATCTCCCCAGTACCATTC11020.5189203443144789No Hit
CAAAGGAGGCTCTATGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA10930.5146823378727091No Hit
CAAAGGAGACTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA10130.47701116950142214No Hit
CAAAGGAGGCACTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA10060.4737149422689345No Hit
AAAATCAAGTTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC8780.4134410728748752No Hit
TAAAGGAGGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA8740.4115575144563109No Hit
AAACTGAAGTTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC8720.41061573524702866No Hit
CAAAGGAGGCTTTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA7810.3677647812246897No Hit
CAAAGGAGGCTCTAGGTTTTCTCTATCATCTCAGTTCAGAGGCAACGAAA7720.3635267747829199No Hit
AAAATGAAGTTTCTGTACCCTTACCTCGAGATATCTCCCCAGTACCATTC7650.36023054755043227No Hit
AAAATGAAGGTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC7130.33574428810909573No Hit
AAAATGAAGTTTTTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC6510.30654913262134825No Hit
CAAAGGAGGCTCTAGCTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA6210.29242244448211563No Hit
AAAATGCAGTTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC5840.2749995291103954No Hit
CAAGGGAGGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA5560.2618146201804449No Hit
CAAAGGTGGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA5520.2599310617618806No Hit
AAAATGAAGTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTCA4850.22838145825092765No Hit
AAAATGAACTTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC4110.19353562750748712No Hit
AAAATGAAGTGTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC4110.19353562750748712No Hit
CAAATGAAGTTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC3530.16622403043830405No Hit
AAAATGAAGTTTCTTTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC3370.15868979676404663No Hit
AAATGAAGTTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTCA3360.15821890715940554No Hit
AAAATGAGGTTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC3300.15539356953155903No Hit
CAAAGGAGGCTCTAGGCTTTGTCTATCATCTCAGTTCAGAGGCAACGAAA2810.1323199789041457No Hit
AAAATGAAGCTTCTGTAGCCTTACCTCGAGATATCTCCCCAGTACCATTC2690.12666930364845264No Hit
CAAAGGAGGCTCTAGGTTTTGTCTATCATCTCAGCTCAGAGGCAACGAAA2620.12337307641596504No Hit
AAAATGAAGTTTCTGTAGCCTTTCCTCGAGATATCTCCCCAGTACCATTC2360.11112994669529674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)