FastQCFastQC Report
Wed 8 May 2024
EGAF00000782644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456245
Total Bases194.2 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT116090.7971872864799535No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT81560.5600705925170558No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC33850.23244715003313318No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC30370.20855007227492625No Hit
ACACAGGGAGGGGAACATCACACACCGGGGCCTGTCAGGGGGTGGGGGGC17070.11721928658982521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)