FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187246
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT6830.3647607959582581No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT5890.314559456543798No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC5180.27664142358181215No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC4580.2445980154449227No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA3950.2109524369011888No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTTGGGGGGTGGGGGGC2370.12657146214071327No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2280.12176495092017987No Hit
ACACAGGGAGGGGAACATTGCAAACCACCATGGCA2230.11909466690877242No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCGGGGGGTGGGGGGC2230.11909466690877242No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCGGGGGGTGGGGGGC2130.11375409888595753No Hit
ACACAGGGAGGGGAACATCACACACCGGGGCCTGTCAGGGGGTGGGGGGC2110.11268598528139454No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTTGGGGGGTGGGGGGC2020.10787947406086112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)