FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184052
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC5560.30208854019516224TruSeq Adapter, Index 12 (100% over 50bp)
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT5400.2933953447938626No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC5100.2770956034164258No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA5040.27383565514093844No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC4560.24775606893703953No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT3480.18907699997826702No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2350.12768130745658834No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTTGGGGGGTGGGGGGC2280.12387803446851976No Hit
ACACAGGGAGGGGAACATCACACACCGGGGCCTGTCAGGGGGTGGGGGGC2260.12279138504335732No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCGGGGGGTGGGGGGC2100.11409818964205767No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTTGGGGGGTGGGGGGC2060.11192489079173276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)