FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137640
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT10940.7948270851496658No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT7530.547079337401918No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA6320.45916884626562043No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC2690.1954373728567277No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC2470.17945364719558268No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1400.1017146178436501No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1380.10026155187445511TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)