FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402680
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19810.4919539088109665No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT12430.30868183172742625No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC10840.26919638422568787No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT10540.26174629979139763No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC9340.2319459620542366No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA7070.17557365650144036No Hit
ACACAGGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTAT6370.15819012615476308TruSeq Adapter, Index 22 (97% over 44bp)
ACACAGGGAGGGGAACATTGCAAACCACCATGGCA4590.11398629184464092No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCGGGGGGTGGGGGGC4290.10653620741035066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)