FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230135
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT29951.3014100419319097No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT10850.4714624025028788No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA7800.33893149673018014No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC5080.22074000043452754No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC4430.19249570904034588No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2450.1064592521780694No Hit
ACACAGGGAGGGGAACATTGCAAACCACCATGGCA2430.10559019705824842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)