FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215549
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT16340.758064291645983No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT11910.5525425773258053No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA7540.34980445281583306No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC5120.23753299713754183TruSeq Adapter, Index 12 (100% over 50bp)
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC4370.20273812450997222No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC3960.1837169274735675No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2240.10392068624767453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)