FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228972
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15000.6551019338609088No Hit
ACACAGGGAGGGGAACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT6950.30353056268888773TruSeq Adapter, Index 6 (97% over 38bp)
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC5170.22579179987072656No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC5090.22229792289013506No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT4960.21662037279667384No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA4090.17862446063274112No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT3470.15154691403315687No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCGGGGGGTGGGGGGC2560.11180406337892844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)