FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204318
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT23001.1256962186395718No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT16050.7855401873550055No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA7130.3489658277782672No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3330.16298123513346843TruSeq Adapter, Index 6 (100% over 50bp)
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC3240.1585763368866179No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC2980.14585107528460536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)