FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153884
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT21321.3854591770424476No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT11310.7349691975773959No Hit
ACACAGGGAGGGGAACATTGCAAACCACCATGGCA5810.3775571209482467No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5240.3405162330066803No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA5160.33531751189207454No Hit
ACCAGCAAGAGGCTTAGACCCAGCTTTCTTGTACAAAGTGGTCCCCGTGA3380.21964596709209533No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2640.17155779678199162TruSeq Adapter, Index 6 (100% over 50bp)
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC2140.13906578981570533No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC1930.1254191468898651No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC1740.1130721842426763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)