FastQCFastQC Report
Wed 8 May 2024
EGAF00000782722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461582
Total Bases59.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT44900.9727415713784333No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT31140.674636359303439No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA25010.5418322204938667No Hit
ACACAGGGAGGGGAACATTGCAAACCACCATGGCA9200.1993145313292113No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8330.18046630934481847No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC8040.17418356868335422No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC7600.16465113457630498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)