FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142866
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT15151.0604342530763093No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT8730.6110621141489228No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA6290.4402727030924083No Hit
ACACAGGGAGGGGAACATTGCAAACCACCATGGCA4870.34087886551033836No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4230.2960816429381378No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC2130.14909075637310487No Hit
ACCAGCAAGAGGCTTAGACCCAGCTTTCTTGTACAAAGTGGTCCCCGTGA2100.14699088656503295No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC2050.14349110355157982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)