FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000782739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34960
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACATTGCAAACCACCATGGCA11153.189359267734554No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11043.1578947368421053No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT4561.3043478260869565No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT2480.7093821510297483No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA1410.4033180778032036No Hit
ACCAGCAAGAGGCTTAGACCCAGCTTTCTTGTACAAAGTGGTCCCCGTGA1000.2860411899313501No Hit
ACACAGGGAGGGGAACATTGCAAACCACCATGGCAAGATCGGAAGAGCAC800.2288329519450801No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC580.16590389016018306No Hit
ACACAGGGAGGGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCT570.16304347826086957TruSeq Adapter, Index 19 (97% over 39bp)
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC450.12871853546910755No Hit
ACACAGGGAGAGGCTTAGACCCAGCTTTCTTGTACAAAGTGGTCCCCGTG380.10869565217391304No Hit
ACACAGGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTAT370.10583524027459955TruSeq Adapter, Index 19 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)