FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000782745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29068
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACATTGCAAACCACCATGGCA16235.583459474336039No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12114.166093298472547No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGT3391.1662309068391359No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGT2090.7190037154258979No Hit
ACACAGGGAGGGGAACATCACACTCTGGGGACTGTTGTGGGGTGGGGGGA1140.3921838447777625No Hit
ACACAGGGAGGGGAACATTGCAAACCACCATGGCAAGATCGGAAGAGCAC1050.36122196229530756No Hit
ACCAGCAAGAGGCTTAGACCCAGCTTTCTTGTACAAAGTGGTCCCCGTGA500.17201045823586072No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAGGGGGTGGGGGGC470.1616898307417091No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGGTGGGGGGC380.13072794825925416No Hit
ACACAGGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTAT310.10664648410623366TruSeq Adapter, Index 16 (97% over 44bp)
ACACAGGGAGGGGAACATCACACACCGGGGCCTGTTGTGGGGTGGGGGGA300.10320627494151645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)