FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000782756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619303
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18287729.52948718155733No Hit
ATGCACTTACTACAAATGCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA9586815.479983142339048No Hit
ACCACATTGGAAAGTCAATGCCAAATGTCCTAGAAGATGAAGTATATGAA566659.149802277721891No Hit
GGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGCT383346.189861828539503No Hit
ACCACAGTGGAAAGTCAATGCCAAATGTCCTAGAAGATGAAGTATATGAA106311.716607218114558No Hit
GGAAGGGGCTGAGGTCACTTAGCAACTGAGTTAAAAGATGGTTCAAGTT98051.5832314715090996No Hit
ATGCACTTAGTACAAATGCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA81561.3169643938427555No Hit
GGAAGGGGCTGAGGTCACCTGAGTTAAAAGATGGTTCAAGTT67701.0931644122505462No Hit
CTGCACTTACTACAAATGCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA66741.077663114824246No Hit
ATGCAATTACTACAAATGCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA62831.0145276221817108No Hit
ATGCACTTACTACCAATGCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA60760.9811029496062509No Hit
ATGCACTTACTACAAATGCAAGCAGGATATGCACACAGTG59330.9580124753149912No Hit
ATGCACTTACTACAAATGCCAGCTCTTAAGGAAGGACAAGGTGGGGAAAA54960.887449277655687No Hit
ATGCACGTACTACAAATGCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA53960.8713020928366243No Hit
ACCACATTGAAAAGTCAATGCCAAATGTCCTAGAAGATGAAGTATATGAA45390.7329207189372569No Hit
CCCACATTGGAAAGTCAATGCCAAATGTCCTAGAAGATGAAGTATATGAA45040.7272692042505849No Hit
ATGCACTTACTACAAATGAAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA41560.6710770010802467No Hit
ATGCCCTTACTACAAATGCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA38280.618114234873721No Hit
ACCACATTGGAAAGTCACTGCCAAATGTCCTAGAAGATGAAGTATATGAA36220.5848510341464518No Hit
ATGCACTTACTACAAGTGCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA33560.5418995225277449No Hit
ACCACATTGGAAAGTCAATGCCCATGAAGCAAACGCTGAT29790.48102463575987847No Hit
ACCACATCGGAAAGTCAATGCCAAATGTCCTAGAAGATGAAGTATATGAA27920.45082940014823114No Hit
ATGCACTTACTACAAATTCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA25940.418857974206487No Hit
ATTCACTTACTACAAATGCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA20760.33521555684374205No Hit
ATGCACTTACTACAAATGCAACCTCTTAAGGAAGGACAAGGTGGGGAAAA19810.3198757312656325No Hit
GGAAGGGGCTGAGTTAAAAGATGGTTCAAGTTAGATCGGAAGAGCACACG19060.3077653426513355No Hit
GGAAGGGGCTGAGGTAAAAGATGGTTCAAGTTAGATCGGAAGAGCACACG17600.28419045281550387No Hit
GGAAGGGGCTGAGGTCACTCCTGAGTTAAAAGATGGTTCAAGTT15550.2510887239364253No Hit
ATGTACTTACTACAAATGCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA13610.21976318538744363No Hit
ATGCACTTACTACAAATGCCAGCAGGATATGCACACAGTG12410.20038656360456836No Hit
ATGCACTTACTACAAATGCAAGCTCTTAAGGAAGGACAAGGCGGGGAAAA12180.19667271109618392No Hit
GGAAGGGGCTGAGGTCACTTCTGAGTTAAAAGATGGTTCAAGTT10740.17342076495673361No Hit
ACCACATTGGAAAGTAAATGCCAAATGTCCTAGAAGATGAAGTATATGAA10450.16873808135920543No Hit
GGAAGGGGCTGAGGTCACTCGTATCACCGACTGCCCATAGAGAGGCTGAGTTAAAAGATGGTTCAAGTT9910.16001860155691155ABI Solid3 Adapter B (95% over 23bp)
CCCACAGTGGAAAGTCAATGCCAAATGTCCTAGAAGATGAAGTATATGAA9090.14677791000528012No Hit
ATGCACTTACAACAAATGCAAGCTCTTAAGGAAGGACAAGGTGGGGAAAA8970.1448402478269926No Hit
ACCACAGTGAAAAGTCAATGCCAAATGTCCTAGAAGATGAAGTATATGAA8420.13595929617650812No Hit
GGAAGGGGCTGAGGTCACTTAGCAACTGAGTTAAAAGATGGTTCAAGTTA8310.13418310584641122No Hit
ACCACATTGGCAAGTCAATGCCAAATGTCCTAGAAGATGAAGTATATGAA6650.1073787790467671No Hit
ATGCACTTACTACAAATGCAAGCTCTTAAGGAAGGACAAGGAGGGGAAAA6620.10689436350219521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)