FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000782760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359972
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAAATCAAAAGTGGCTTGTGTGTCCCAGGAGAATCAGTGCGTATACA9233425.650328358872354No Hit
CATAGGGCACCACCACACTATGTCGAAAAGTGTTTCTGTCATCCAAATAC8190722.753714177769382No Hit
CATTCATGGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA6067016.85408865133955No Hit
CATTCATGGTGGGGAGTGGGGCGGAAGAGATCACAGGTGGGC111303.091907148333759No Hit
CATTCATGGTGGGGAGTGGGAAGAGCACACGTCTGAACTCCAGTCACCAG33770.9381285211071972TruSeq Adapter, Index 7 (97% over 34bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29210.811452001822364No Hit
CATTCATGGTGGGGAGTGGGCGGAAGAGATCACAGGTGGGC26500.7361683686508951No Hit
CATTCATGGTGGGGAGTGGTGGGGGGGCGTCGGGCGGAAGAGATCACAGGTGGGC25470.707555032058049No Hit
CATTCATGGTGGGGAGTGGCGCGGTGGGGGGGCGTCGGGCGGAAGAGATCACAGGTGGGC23790.6608847354794262No Hit
GCTGAATCAAAAGTGGCTTGTGTGTCCCAGGAGAATCAGTGCGTATACAG13240.367806384941051No Hit
GCTGAAATCAAAGTGGCTTGTGTGTCCCAGGAGAATCAGTGCGTATACAG12680.3522496194148434No Hit
CATTCATGGTGGGGAGTGGGGGGGCGTCGGGCGGAAGAGATCACAGGTGGGC11420.31724689698087627No Hit
CATTCATGGTGGGGAGTCGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA10320.28668896469725424No Hit
CATTCATGGTGGGGAGTCGGGCGGAAGAGATCACAGGTGGGC9990.2775215850121676No Hit
CATTCAGGGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA9040.25113064349449404No Hit
CATTCATGGTGGGCAGTCGGAAGAGCACACGTCTGAACTCCAGTCACCAG9040.25113064349449404TruSeq Adapter, Index 7 (100% over 34bp)
CATTCATGGGGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA7980.2216839087484582No Hit
CATTCATGGTGGGGAGCGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA7520.20890513706621627No Hit
CATTCATGGTGGGGAGTGGGAAGAGATCACAGGTGGGC7320.20334914937828497No Hit
CATTCATGGTGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCAC6520.18112519862655985No Hit
CAGTCATGGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA6430.17862500416699076No Hit
CATTCATGGTGGGGAGTGGGGCCGGAAGAGATCACAGGTGGGC5810.1614014423344038No Hit
CATTCATGGTGGGGAGTGGAAGAGCACACGTCTGAACTCCAGTCACCAGA5350.14862267065216184TruSeq Adapter, Index 7 (100% over 33bp)
GCTGAAATAAAAGTGGCTTGTGTGTCCCAGGAGAATCAGTGCGTATACAG5190.14417788050181682No Hit
CATTCATGGTGGGGGGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA5100.14167768604224773No Hit
CATCATGGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCAC5050.1402886891202649No Hit
CATTCATGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCAC4650.12917671374440234No Hit
GCTGAAACAAAAGTGGCTTGTGTGTCCCAGGAGAATCAGTGCGTATACAG4370.12139833098129854No Hit
CATTCACGGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA4370.12139833098129854No Hit
CATTCATGGTGGGGAGTGGAAGAGATCACAGGTGGGC4300.1194537352905226No Hit
AATTCATGGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA4240.11778693898414322No Hit
GTTGAAATCAAAAGTGGCTTGTGTGTCCCAGGAGAATCAGTGCGTATACA4210.11695354083095352No Hit
CATTCATGGTGGGGAGTCGGAAGAGCACACGTCTGAACTCCAGTCACCAG4180.11612014267776384TruSeq Adapter, Index 7 (100% over 34bp)
CATAGGGCACCACCACACTATGTCGAAAAGCGTTTCTGTCATCCAAATAC4170.11584234329336726No Hit
CATTCATGGTGGGGAGTGGCGGAAGAGATCACAGGTGGGC4100.11389774760259132No Hit
CATTCATGCTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA4020.1116753525274188No Hit
CATCCATGGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA3960.11000855622103942No Hit
CATTCATGGTGGGGCGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA3860.10723056237707378No Hit
CATTCGTGGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA3850.1069527629926772No Hit
CATTCATCGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA3800.1055637660706944No Hit
GCTGAAATCAAAAGTGGCTTGTGTGTCCCAGGAGAATCAGTGCGCATACA3730.10361917037991844No Hit
GATTCATGGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA3710.1030635716111253No Hit
GCTGAAATCAAAAGTGGCTTGTGTGTCCCAGGAGAATCAGCGCGTATACA3680.10223017345793561No Hit
CATTGATGGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA3660.10167457468914248No Hit
CATGCATGGTGGGGAGTGGCTGGCATGGCCAGTGCTGGGAGTGATGTCCA3600.1000077783827631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)