FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000782767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000782767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69303
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT5028272.55385769735798No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGCGGTATCCACATCCTCTTCCT4740.6839530756244319No Hit
TACCTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT4210.6074773097845692No Hit
TCCCTGTTCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCTC3890.5613032624850295No Hit
TCCTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCTC3500.5050286423387156No Hit
TCCCTGTTCCCACTCATACAGGCCTTGGGAGGTATCCACATCCTCTTCCT3310.47761280175461385No Hit
TCACTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT3060.44153932730184836No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2960.4271099375207423No Hit
TCTCTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT2830.40835173080530424No Hit
TCCCTGTTCCCACTAATACAGGACTTGGGAGGTATCCACATCCTCTTCCT2470.35640592759332207No Hit
TCCCTGTTCTCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT2460.35496298861521147No Hit
TCCCTGTTCACACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT2440.3520771106589903No Hit
CCCTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCTC2220.3203324531405567No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGAGGCATCCACATCCTCTTCCT1940.2799301617534594No Hit
TCCCTGTTCCCACTCTTACAGGACTTGGGAGGTATCCACATCCTCTTCCT1910.2756013448191276No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGTGGTATCCACATCCTCTTCCT1910.2756013448191276No Hit
TCCCTGTTCCCACTCATACAGGGCTTGGGAGGTATCCACATCCTCTTCCT1740.25107138219124714No Hit
TCCCTGTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCTC1740.25107138219124714No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGAGGTCTCCACATCCTCTTCCT1620.23375611445391975No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGAGGTATCAACATCCTCTTCCT1620.23375611445391975No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCC1600.2308702364976985No Hit
TCCCTGTTGCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT1580.22798435854147728No Hit
TCCCTGTTCCCACTCATCAGGACTTGGGAGGTATCCACATCCTCTTCCTC1540.2222126026290348No Hit
TCCCGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCTC1440.20778321284792867No Hit
TCCCTGTTCCCACTCATTCAGGACTTGGGAGGTATCCACATCCTCTTCCT1420.20489733489170744No Hit
GCCCTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT1360.19623970102304375No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGGGGTATCCACATCCTCTTCCT1360.19623970102304375No Hit
TCCCTGTTCCCACTCATACGGGACTTGGGAGGTATCCACATCCTCTTCCT1320.19046794511060125No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGAGGAATCCACATCCTCTTCCT1310.18902500613249065No Hit
TCCTTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT1280.1846961891981588No Hit
TCCCTGTTCCCGCTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT1270.18325325022004818No Hit
TCCCTGTTCCCAATCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT1250.18036737226382696No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGAGGTATACACATCCTCTTCCT1190.17170973839516326No Hit
TCCCTGTTCCCACTATACAGGACTTGGGAGGTATCCACATCCTCTTCCTC1180.17026679941705264No Hit
TCCCTGATCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT1140.1644950435046102No Hit
TCCCTGTTCCCCTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCTC1110.16016622657027835No Hit
TCCCTGTTCCCACTCATAAAGGACTTGGGAGGTATCCACATCCTCTTCCT1090.15728034861405712No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCCTCCT1080.15583740963594647No Hit
TCCCTGTTCCCATCATACAGGACTTGGGAGGTATCCACATCCTCTTCCTC1020.1471797757672828No Hit
TCCCTTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCTC980.14140801985484036No Hit
TCCCTGTTCCCACTCTACAGGACTTGGGAGGTATCCACATCCTCTTCCTC980.14140801985484036No Hit
TCCCTGTTCCAACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT970.1399650808767297No Hit
TCCCTGTTCCCACCATACAGGACTTGGGAGGTATCCACATCCTCTTCCTC970.1399650808767297No Hit
TCCCTGTTCCCACTCATACAGGAATTGGGAGGTATCCACATCCTCTTCCT950.13707920292050849No Hit
CCCCTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT910.13130744700806604No Hit
TCCCGGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT910.13130744700806604No Hit
TCCCTGTTCCTACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT900.12986450802995542No Hit
TCCCTGTTCCCACTCATACCGGACTTGGGAGGTATCCACATCCTCTTCCT880.1269786300737342No Hit
TCCCTGTTCCCCCTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT850.12264981313940233No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGAGGTACCCACATCCTCTTCCT810.11687805722695987No Hit
AATTGGCATTCCGACACTGGCAAGCATGGACCAGAGACTGGATGCAGCGC800.11543511824884925No Hit
TCCCTGTTCCCACTCATATAGGACTTGGGAGGTATCCACATCCTCTTCCT790.11399217927073864No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGAGGTATCCACATCCCCTTCCT790.11399217927073864No Hit
TCCCTGTTCCCACTCATCCAGGACTTGGGAGGTATCCACATCCTCTTCCT780.11254924029262803No Hit
TCCCTGTTCCCACTCATACAGGACCTGGGAGGTATCCACATCCTCTTCCT750.10822042335829618No Hit
TCCCTGTTCCCACTCATACAGAACTTGGGAGGTATCCACATCCTCTTCCT740.10677748438018557No Hit
TCCCTGTTCCCACTCATACAGGACTTGGGAGGTATCCACACCCTCTTCCT730.10533454540207494No Hit
TCCCTGCTCCCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCT700.10100572846774308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)